TY  - JOUR
A1  - Fischer, Manuel
A1  - Grininger, Martin
T1  - Strategies in megasynthase engineering – fatty acid synthases (FAS) as model proteins
T2  - Beilstein journal of organic chemistry
N2  - Megasynthases are large multienzyme proteins that produce a plethora of important natural compounds by catalyzing the successive condensation and modification of precursor units. Within the class of megasynthases, polyketide synthases (PKS) are responsible for the production of a large spectrum of bioactive polyketides (PK), which have frequently found their way into therapeutic applications. Rational engineering approaches have been performed during the last 25 years that seek to employ the "assembly-line synthetic concept" of megasynthases in order to deliver new bioactive compounds. Here, we highlight PKS engineering strategies in the light of the newly emerging structural information on megasynthases, and argue that fatty acid synthases (FAS) are and will be valuable objects for further developing this field.
KW  - fatty acid synthases
KW  - megasynthases
KW  - metabolic enzyme engineering
KW  - polyketide synthases
KW  - protein design
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44454
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-444542
SN  - 1860-5397
N1  - This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 13
SP  - 1204
EP  - 1211
PB  - Beilstein-Institut zur Förderung der Chemischen Wissenschaften
CY  - Frankfurt, Main
ER  -