TY  - JOUR
A1  - Zettler, Joachim
A1  - Simone, Eppmann
A1  - Busche, Alena
A1  - Dikovskaya, Dina
A1  - Dötsch, Volker
A1  - Mootz, Henning D.
A1  - Sonntag, Tim
T1  - Splicefinder : a fast and easy screening method for active protein trans-splicing positions
T2  - PLoS One
N2  - Split intein enabled protein trans-splicing (PTS) is a powerful method for the ligation of two protein fragments, thereby paving the way for various protein modification or protein function control applications. PTS activity is strongly influenced by the amino acids directly flanking the splice junctions. However, to date no reliable prediction can be made whether or not a split intein is active in a particular foreign extein context. Here we describe SPLICEFINDER, a PCR-based method, allowing fast and easy screening for active split intein insertions in any target protein. Furthermore we demonstrate the applicability of SPLICEFINDER for segmental isotopic labeling as well as for the generation of multi-domain and enzymatically active proteins.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/31580
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-315808
SN  - 1932-6203
N1  - Copyright: © 2013 Zettler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (9):e72925
PB  - PLoS
CY  - Lawrence, Kan.
ER  -