TY  - JOUR
A1  - Mobini, Sahba
A1  - Leppik, Liudmila
A1  - Parameswaran, Vishnu Thottakkattumana
A1  - Barker, John Howard
T1  - In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells
T2  - PeerJ
N2  - Background: Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation.
Methods: In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points.
Results: Results showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES.
Discussion: This study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.
KW  - Direct current electrical stimulation
KW  - Bone marrow-derived mesenchymal stem cells
KW  - Adipose tissue-derived mesenchymal stem cells
KW  - Bone tissue engineering
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44227
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-442273
SN  - 2167-8359
N1  - Distributed under Creative Commons CC-BY 4.0
VL  - 5
IS  - e2821
SP  - 1
EP  - 15
PB  - PeerJ, Inc.
CY  - London [u. a.]
ER  -