TY  - JOUR
A1  - Patra, Chinmoy
A1  - Diehl, Florian
A1  - Ferrazzi, Fulvia
A1  - Amerongen, Machteld J. van
A1  - Novoyatleva, Tatyana
A1  - Schäfer, Liliana
A1  - Mühlfeld, Christian
A1  - Jungblut, Benno
A1  - Engel, Felix B.
T1  - Nephronectin regulates atrioventricular canal differentiation via Bmp4-Has2 signaling in zebrafish
T2  - Development
N2  - The extracellular matrix is crucial for organogenesis. It is a complex and dynamic component that regulates cell behavior by modulating the activity, bioavailability and presentation of growth factors to cell surface receptors. Here, we determined the role of the extracellular matrix protein Nephronectin (Npnt) in heart development using the zebrafish model system. The vertebrate heart is formed as a linear tube in which myocardium and endocardium are separated by a layer of extracellular matrix termed the cardiac jelly. During heart development, the cardiac jelly swells at the atrioventricular (AV) canal, which precedes valve formation. Here, we show that Npnt expression correlates with this process. Morpholino-mediated knockdown of Npnt prevents proper valve leaflet formation and trabeculation and results in greater than 85% lethality at 7 days post-fertilization. The earliest observed phenotype is an extended tube-like structure at the AV boundary. In addition, the expression of myocardial genes involved in cardiac valve formation (cspg2, fibulin 1, tbx2b, bmp4) is expanded and endocardial cells along the extended tube-like structure exhibit characteristics of AV cells (has2, notch1b and Alcam expression, cuboidal cell shape). Inhibition of has2 in npnt morphants rescues the endocardial, but not the myocardial, expansion. By contrast, reduction of BMP signaling in npnt morphants reduces the ectopic expression of myocardial and endocardial AV markers. Taken together, our results identify Npnt as a novel upstream regulator of Bmp4-Has2 signaling that plays a crucial role in AV canal differentiation.
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/26380
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-263803
SN  - 1477-9129
SN  - 0950-1991
N1  - Copyright © 2011. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.
VL  - 138
SP  - 4499
EP  - 4509
PB  - The Company of Biologists Ltd
CY  - Cambridge
ER  -