TY  - JOUR
A1  - Gaynes, John A.
A1  - Otsuna, Hideo
A1  - Campbell, Douglas S.
A1  - Manfredi, John P.
A1  - Levine, Edward M.
A1  - Chien, Chi-Bin
T1  - The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo
T2  - PLoS One
N2  - Attractive growth cone turning requires Igf2bp1-dependent local translation of β-actin mRNA in response to external cues in vitro. While in vivo studies have shown that Igf2bp1 is required for cell migration and axon terminal branching, a requirement for Igf2bp1 function during axon outgrowth has not been demonstrated. Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the β-actin 3’UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. Igf2bp1 knockdown reduced RGC axonal outgrowth and tectal coverage and retinal cell survival. RGC-specific expression of a phosphomimetic Igf2bp1 reduced the density of axonal projections in the optic tract while sparing RGCs, demonstrating for the first time that Igf2bp1 is required during axon outgrowth in vivo. Therefore, regulation of local translation mediated by Igf2bp proteins may be required at all stages of axon development.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/39283
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-392832
SN  - 1932-6203
N1  - Copyright: © 2015 Gaynes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
VL  - 10
IS  - (9): e0134751
SP  - 1
EP  - 20
PB  - PLoS
CY  - Lawrence, Kan.
ER  -