TY  - JOUR
A1  - Martin, Roman
A1  - Hackert, Philipp
A1  - Ruprecht, Maike
A1  - Simm, Stefan
A1  - Brüning, Lukas
A1  - Mirus, Oliver
A1  - Sloan, Katherine E.
A1  - Kudla, Grzegorz
A1  - Schleiff, Enrico
A1  - Bohnsack, Markus T.
T1  - A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase
T2  - RNA
N2  - Ribosome biogenesis in yeast requires 75 small nucleolar RNAs (snoRNAs) and a myriad of cofactors for processing, modification, and folding of the ribosomal RNAs (rRNAs). For the 19 RNA helicases implicated in ribosome synthesis, their sites of action and molecular functions have largely remained unknown. Here, we have used UV cross-linking and analysis of cDNA (CRAC) to reveal the pre-rRNA binding sites of the RNA helicase Rok1, which is involved in early small subunit biogenesis. Several contact sites were identified in the 18S rRNA sequence, which interestingly all cluster in the “foot” region of the small ribosomal subunit. These include a major binding site in the eukaryotic expansion segment ES6, where Rok1 is required for release of the snR30 snoRNA. Rok1 directly contacts snR30 and other snoRNAs required for pre-rRNA processing. Using cross-linking, ligation and sequencing of hybrids (CLASH) we identified several novel pre-rRNA base-pairing sites for the snoRNAs snR30, snR10, U3, and U14, which cluster in the expansion segments of the 18S rRNA. Our data suggest that these snoRNAs bridge interactions between the expansion segments, thereby forming an extensive interaction network that likely promotes pre-rRNA maturation and folding in early pre-ribosomal complexes and establishes long-range rRNA interactions during ribosome synthesis.
KW  - RNA helicase
KW  - small nucleolar RNA
KW  - ribosome biogenesis
KW  - RNA–protein complex
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/34418
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-344182
SN  - 1469-9001
SN  - 1355-8382
N1  - This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
VL  - 20
IS  - 8
SP  - 1173
EP  - 1182
PB  - Laboratory Press
CY  - Cold Spring Harbor, NY
ER  -