TY  - JOUR
A1  - Jesus, Vanessa de
A1  - Qureshi, Nusrat
A1  - Warhaut, Sven
A1  - Bains, Jasleen Kaur
A1  - Dietz, Marina
A1  - Heilemann, Mike
A1  - Schwalbe, Harald
A1  - Fürtig, Boris
T1  - Switching at the ribosome: riboswitches need rProteins as modulators to regulate translation
T2  - Nature Communications
N2  - Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Their mechanism is considered as an RNA-only gene-regulatory system inducing a ligand-dependent shift of the population of functional ON- and OFF-states. The interaction of riboswitches with the translation machinery remained unexplored. For the adenine-sensing riboswitch from Vibrio vulnificus we show that ligand binding alone is not sufficient for switching to a translational ON-state but the interaction of the riboswitch with the 30S ribosome is indispensable. Only the synergy of binding of adenine and of 30S ribosome, in particular protein rS1, induces complete opening of the translation initiation region. Our investigation thus unravels the intricate dynamic network involving RNA regulator, ligand inducer and ribosome protein modulator during translation initiation.
KW  - RNA
KW  - Solution-state NMR
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79968
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-799680
SN  - 2041-1723
N1  - The work was supported by funds from DFG in collaborative research center 902: “Molecular principles of RNA-based regulation” and in Graduate College GRK1986 (CLIC). Work at BMRZ is supported by the state of Hesse (HMWK). Open Access funding enabled and organized by Projekt DEAL.
VL  - 12
IS  - art. 4723
SP  - 1
EP  - 7
PB  - Nature Publishing Group UK
CY  - [London]
ER  -