TY  - JOUR
A1  - Novakovic, Mihajlo
A1  - Kupče, Ēriks
A1  - Scherf, Tali
A1  - Oxenfarth, Andreas
A1  - Schnieders, Robbin
A1  - Grün, J. Tassilo
A1  - Wirmer-Bartoschek, Julia
A1  - Richter, Christian
A1  - Schwalbe, Harald
A1  - Frydman, Lucio
T1  - Magnetization transfer to enhance NOE cross-peaks among labile protons: applications to imino–imino sequential walks in SARS-CoV-2-derived RNAs
T2  - Angewandte Chemie
N2  - 2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent-driven exchanges to enhance NOE correlations between exchangeable and non-exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross-peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS-CoV-2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.
KW  - 2D NMR spectroscopy
KW  - CEST
KW  - NOESY
KW  - RNA
KW  - SARS-CoV-2
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62229
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-622290
SN  - 1521-3773
N1  - This work was supported by the EU Horizon 2020 program (FET-OPEN Grant 828946, PATHOS), Israel Science Foundation Grants 965/18 and 3572/20, and the Perlman Family Foundation. HS was supported by the Goethe Corona Funds, EU-supported iNEXT-discovery, and by DFG-funded collaborative research center 902. Work at BMRZ is supported by the state of Hesse. Joint support to L.F., H.S. was given by the German-Israel Foundation (grant G-1501-302). Open access funding enabled and organized by Projekt DEAL.
VL  - 60
IS  - 21
SP  - 11884
EP  - 11891
PB  - Wiley-VCH
CY  - Weinheim
ER  -