TY  - JOUR
A1  - Wolfram-Schauerte, Maik
A1  - Moskalchuk, Anastassiya
A1  - Pozhydaieva, Nadiia
A1  - Ramı́rez Rojas, Adán Andrés
A1  - Schindler, Daniel
A1  - Kaiser, Stefanie
A1  - Paczia, Nicole
A1  - Höfer, Katharina
T1  - T4 phage RNA is NAD-capped and alters the NAD-cap epitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme
T2  - bioRxiv
N2  - Nicotinamide adenine dinucleotide (NAD) serves as a cap-like structure on cellular RNAs (NAD-RNAs) in all domains of life including the bacterium Escherichia coli. NAD also acts as a key molecule in phage-host interactions, where bacterial immune systems deplete NAD to abort phage infection. Nevertheless, NAD-RNAs have not yet been identified during phage infections of bacteria and the mechanisms of their synthesis and degradation are unknown in this context. The T4 phage that specifically infects E. coli presents an important model to study phage infections, but a systematic analysis of the presence and dynamics of NAD-RNAs during T4 phage infection is lacking. Here, we investigate the presence of NAD-RNAs during T4 phage infection in a dual manner. By applying time-resolved NAD captureSeq, we identify NAD-capped host and phage transcripts and their dynamic regulation during phage infection. We provide evidence that NAD-RNAs are – as reported earlier – generated by the host RNA polymerase by initiating transcription with NAD at canonical transcription start sites. In addition, we characterize NudE.1 – a T4 phage-encoded Nudix hydrolase – as the first phage-encoded NAD-RNA decapping enzyme. T4 phages carrying inactive NudE.1 display a delayed lysis phenotype. This study investigates for the first time the dual epitranscriptome of a phage and its host, thereby introducing epitranscriptomics as an important field of phage research.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83597
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-835978
UR  - https://www.biorxiv.org/content/10.1101/2024.04.04.588121v1
IS  - 2024.04.04.588121 Version 1
PB  - bioRxiv
ER  -