TY  - INPR
A1  - Parra-Rojas, César
A1  - Fürtig, Boris
A1  - Schwalbe, Harald
A1  - Hernández-Vargas, Esteban A.
T1  - Quantitative modeling of the function of kinetically driven transcriptional riboswitches
T2  - bioRxiv
N2  - We propose a generalized modeling framework for the kinetic mechanisms of transcriptional riboswitches. The formalism accommodates time-dependent transcription rates and changes of metabolite concentration and permits incorporation of variations in transcription rate depending on transcript length. We derive explicit analytical expressions for the fraction of transcripts that determine repression or activation of gene expression, pause site location and its slowing down of transcription for the case of the (2’dG)-sensing riboswitch from Mesoplasma florum. Our modeling challenges the current view on the exclusive importance of metabolite binding to transcripts containing only the aptamer domain. Numerical simulations of transcription proceeding in a continuous manner under time-dependent changes of metabolite concentration further suggest that rapid modulations in concentration result in a reduced dynamic range for riboswitch function regardless of transcription rate, while a combination of slow modulations and small transcription rates ensures a wide range of finely tuneable regulatory outcomes.
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72651
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-726512
IS  - 821884
ER  -