TY  - JOUR
A1  - Sun, Chao
A1  - Nold, Andreas
A1  - Fusco, Claudia Mariateresa
A1  - Rangaraju, Vidhya
A1  - Tchumatchenko, Tatjana
A1  - Heilemann, Mike
A1  - Schuman, Erin
T1  - The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity
T2  - Science advances
N2  - To supply proteins to their vast volume, neurons localize mRNAs and ribosomes in dendrites and axons. While local protein synthesis is required for synaptic plasticity, the abundance and distribution of ribosomes and nascent proteins near synapses remain elusive. Here, we quantified the occurrence of local translation and visualized the range of synapses supplied by nascent proteins during basal and plastic conditions. We detected dendritic ribosomes and nascent proteins at single-molecule resolution using DNA-PAINT and metabolic labeling. Both ribosomes and nascent proteins positively correlated with synapse density. Ribosomes were detected at ~85% of synapses with ~2 translational sites per synapse; ~50% of the nascent protein was detected near synapses. The amount of locally synthesized protein detected at a synapse correlated with its spontaneous Ca2+ activity. A multifold increase in synaptic nascent protein was evident following both local and global plasticity at respective scales, albeit with substantial heterogeneity between neighboring synapses.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62739
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-627395
SN  - 2375-2548
N1  - C.S. is supported by an EMBO long-term postdoctoral fellowship (EMBO ALTF 860-2018) and HFSP Cross-Disciplinary Fellowship (LT000737/2019-C). C.S. and A.N. are both supported by Joachim Herz Stiftung Add-on Fellowship for Interdisciplinary Life Science (project number 850027). T.T. is funded by the Max Planck Society, the DFG CRC1080: Molecular and Cellular Mechanisms of Neural Homeostasis, and the fellowship of the Behrens-Weise-Foundation. M.H. is funded by the German Science Foundation (grants SFB 807, SFB902, HE 6166/11-1, and EXC115) and the Bundesministerium für Bildung und Forschung (BMBF:eBio). E.M.S. is funded by the Max Planck Society, an Advanced Investigator award from the European Research Council (grant 743216), DFG CRC 1080: Molecular and Cellular Mechanisms of Neural Homeostasis, and DFG CRC 902: Molecular Principles of RNA-based Regulation.
VL  - 7
IS  - 38, eabj0790
SP  - 1
EP  - 13
PB  - Assoc.
CY  - Washington, DC [u.a.]
ER  -