TY  - JOUR
A1  - Kahl, Günter
A1  - Wechselberger, Manfred
T1  - Activation of chromatin-bound DNA-dependent RNA polymerase (E.C. 2.7.7.6.) in plant storage tissue slices
T2  - Zeitschrift für Naturforschung, C
N2  - Chromatin, RNA Polymerase, Potato Tuber Tissue, Aging Phenomenon The synthesis of RNA by chromatin-bound RNA polymerase (E.C. 2.7.7.6.) from white potato tubers proceeds at a low rate, which is enhanced after slicing the tissue, however. Concomitantly DNA template availability as measured with saturating amounts of Escherichia coli polymerase is diminished drastically. Nearest neighbor frequency analysis proved that the RNA synthesized on chromatin of intact tubers is different from that synthesized on chromatin of sliced tissue.
The RNA polymerase of white potato tubers is dependent on all four ribonucleoside triphos­ phates and a divalent metal ion such as Mg2+ or Mn2+ and totally inhibited by the presence of pyrophosphate. Actinomycin D blocks the formation of the RNA product, which could be shown to be a heteropolymer by nearest neighbour frequency technique. The Km of the chromatin-bound enzyme with regard to ATP, GTP, CTP and UTP was 5.1 X10-5 M, 1.6X10-5 M, 0.9X10-5 M and 0.45 X 10-5M/1 respectively, α-amanitin inhibits the overall activity to about 50%, which indicates the presence of equal amounts of polymerase I and polymerase If.
KW  - Chromatin
KW  - RNA Polymerase
KW  - Potato Tuber Tissue
KW  - Aging Phenomenon
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72033
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-720330
SN  - 0939-5075
SN  - 1865-7125
VL  - 32.1977
IS  - 3-4
SP  - 229
EP  - 235
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -