TY  - JOUR
A1  - Vonck, Janet
A1  - Pisa, Kim Yvonne
A1  - Morgner, Nina
A1  - Brutschy, Bernd
A1  - Müller, Volker
T1  - Three-dimensional structure of A1A0 ATP synthase from the hyperthermophilic archaeon Pyrococcus furiosus by electron microscopy
T2  - Journal of biological chemistry
N2  - The archaeal ATP synthase is a multisubunit complex that consists of a catalytic A(1) part and a transmembrane, ion translocation domain A(0). The A(1)A(0) complex from the hyperthermophile Pyrococcus furiosus was isolated. Mass analysis of the complex by laser-induced liquid bead ion desorption (LILBID) indicated a size of 730 +/- 10 kDa. A three-dimensional map was generated by electron microscopy from negatively stained images. The map at a resolution of 2.3 nm shows the A(1) and A(0) domain, connected by a central stalk and two peripheral stalks, one of which is connected to A(0), and both connected to A(1) via prominent knobs. X-ray structures of subunits from related proteins were fitted to the map. On the basis of the fitting and the LILBID analysis, a structural model is presented with the stoichiometry A(3)B(3)CDE(2)FH(2)ac(10).
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76446
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-764464
SN  - 0021-9258
VL  - 284.2009
IS  - 15
SP  - 10110
EP  - 10119
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -