TY  - JOUR
A1  - Hahn, Alexander
A1  - Stevanovic, Mara
A1  - Mirus, Oliver
A1  - Schleiff, Enrico
T1  - The TolC-like protein HgdD of the cyanobacterium Anabaena sp. PCC 7120 is involved in secondary metabolite export and antibiotic resistance
T2  - Journal of biological chemistry
N2  - The role of TolC has largely been explored in proteobacteria, where it functions as a metabolite and protein exporter. In contrast, little research has been carried out on the function of cyanobacterial homologues, and as a consequence, not much is known about the mechanism of cyanobacterial antibiotic uptake and metabolite secretion in general. It has been suggested that the TolC-like homologue of the filamentous, heterocyst-forming cyanobacterium Anabaena sp. PCC 7120, termed heterocyst glycolipid deposition protein D (HgdD), is involved in both protein and lipid secretion. To describe its function in secondary metabolite secretion, we established a system to measure the uptake of antibiotics based on the fluorescent molecule ethidium bromide. We analyzed the rate of porin-dependent metabolite uptake and confirmed the functional relation between detoxification and the action of HgdD. Moreover, we identified two major facilitator superfamily proteins that are involved in this process. It appears that anaOmp85 (Alr2269) is not required for insertion or assembly of HgdD, because an alr2269 mutant does not exhibit a phenotype similar to the hgdD mutant. Thus, we could assign components of the metabolite efflux system and describe parameters of detoxification by Anabaena sp. PCC 7120.
KW  - Cyanobacteria
KW  - Membrane Biogenesis
KW  - Membrane Proteins
KW  - Secretion
KW  - Transport
KW  - Omp85-independent OMP Insertion
KW  - TolC
KW  - Antibiotics and Metabolite Export
KW  - Porin
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76641
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-766415
SN  - 0021-9258
VL  - 287.2012
IS  - 49
SP  - 41126
EP  - 41138
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -