TY  - JOUR
A1  - Woenckhaus, Christoph
A1  - Duchmann, Helmut
T1  - Reactions of Essential Sulfhydryl Residues of Dehydrogenases with Nicotinamide-(S-Methylmercury-Thioinosine) Dinucleotide
T2  - Zeitschrift für Naturforschung, C
N2  - Sulfhydryl Groups, Methylmercury Containing Inactivator, Coenzyme Analogue Nicotinamide-(S-methylmercury-thioinosine) dinucleotide was formed by reaction of nicotin­ amide-(6-thiopurine) dinucleotide with methylmercury chloride. The compound exhibits coenzyme properties in the test with LDH (Km=1.5 × 10-4 м , Vmax=12500) and LADH (Km=1.7 × 10-4 м, Vmax=27) and inactivates YADH and GAPDH. From incubations with LDH and LADH the mercury containing coenzyme could be regained by column chromatography. The compound seems to be qualified for the X-ray structure analysis of the coenzyme-enzyme complex for some dehyrogenases based on the proportion of the heavy metal.
KW  - Sulfhydryl Groups
KW  - Methylmercury Containing Inactivator
KW  - Coenzyme Analogue
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72108
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-721082
SN  - 0939-5075
SN  - 1865-7125
VL  - 30.1975
IS  - 9-10
SP  - 562
EP  - 564
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -