TY  - JOUR
A1  - Baer, Patrick C.
A1  - Sann, Julia
A1  - Dücker, Ruth Pia
A1  - Ullrich, Evelyn
A1  - Geiger, Helmut
A1  - Bader, Peter
A1  - Zielen, Stefan
A1  - Schubert, Ralf
T1  - Tracking of infused mesenchymal stem cells in injured pulmonary tissue in Atm-deficient mice
T2  - Cells
N2  - Pulmonary failure is the main cause of morbidity and mortality in the human chromosomal instability syndrome Ataxia-telangiectasia (A-T). Major phenotypes include recurrent respiratory tract infections and bronchiectasis, aspiration, respiratory muscle abnormalities, interstitial lung disease, and pulmonary fibrosis. At present, no effective pulmonary therapy for A-T exists. Cell therapy using adipose-derived mesenchymal stromal/stem cells (ASCs) might be a promising approach for tissue regeneration. The aim of the present project was to investigate whether ASCs migrate into the injured lung parenchyma of Atm-deficient mice as an indication of incipient tissue damage during A-T. Therefore, ASCs isolated from luciferase transgenic mice (mASCs) were intravenously transplanted into Atm-deficient and wild-type mice. Retention kinetics of the cells were monitored using in vivo bioluminescence imaging (BLI) and completed by subsequent verification using quantitative real-time polymerase chain reaction (qRT-PCR). The in vivo imaging and the qPCR results demonstrated migration accompanied by a significantly longer retention time of transplanted mASCs in the lung parenchyma of Atm-deficient mice compared to wild type mice. In conclusion, our study suggests incipient damage in the lung parenchyma of Atm-deficient mice. In addition, our data further demonstrate that a combination of luciferase-based PCR together with BLI is a pivotal tool for tracking mASCs after transplantation in models of inflammatory lung diseases such as A-T.
KW  - tracking
KW  - mesenchymal stromal/stem cells
KW  - bio imaging
KW  - bioluminescence
KW  - qRT-PCR
KW  - Ataxia telangiectasia
KW  - Atm
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55438
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-554387
SN  - 2073-4409
N1  - © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 9
IS  - 6, art. 1444
SP  - 1
EP  - 11
PB  - MDPI
CY  - Basel
ER  -