TY  - JOUR
A1  - Klebes, Anna
A1  - Kittel, Anna-Sophia
A1  - Verboket, René
A1  - Stetten, Felix von
A1  - Früh, Susanna Maria
T1  - Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
T2  - Sensors and actuators. B, Chemical
N2  - The detection of multiple biomolecule classes in one go is highly desirable for a wide variety of areas, and in particular for point-of-care diagnostics. For example, wound infections are a major problem for patient’s health and cause huge efforts in our healthcare system. In this regard, monitoring infected wounds through simultaneous detection of pathogens via nucleic acid analysis and detection of local inflammation biomarkers is key in order to enable a personalized therapy, improve the clinical outcome and thus, leading to a reduction of overall healthcare costs. In this regard, wound exudate offers an attractive sample material which can be collected in a non-invasive manner. Here, we report the development of a Multianalyte-Assay detecting inflammation biomarkers and pathogen DNA simultaneously from one sample within 35 min. Protein-compatible amplification and labeling transforms nucleic acid information into the measurement principle for protein detection. The combination with rapid detection via lateral flow immunoassay enables a fast and straightforward analysis of multiple biomolecule classes using identical assay conditions. To demonstrate the feasibility of the Multianalyte-Assay, the proinflammatory cytokine interleukin-6 (IL-6) and gDNA of the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa) are used. The detection limits of 4 ng/mL IL-6 and 70 copies/reaction P. aeruginosa gDNA meet the clinically relevant range and thus, having tremendous potential to improve the wound management at the point-of-care.
KW  - Multianalyte detection
KW  - Lateral flow immunoassay
KW  - Isothermal amplification
KW  - Point-of-care wound diagnostics
KW  - Pseudomonas aeruginosa
KW  - Interleukin-6
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78455
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-784553
SN  - 0925-4005
VL  - 355.2022
IS  - 131283
PB  - Elsevier
CY  - Amsterdam
ER  -