TY  - JOUR
A1  - Bein, Julia
A1  - Flinner, Nadine
A1  - Häupl, Björn
A1  - Mathur, Aastha
A1  - Schneider, Olga
A1  - Abu-Ayyad, Marwa
A1  - Hansmann, Martin-Leo
A1  - Piel, Matthieu
A1  - Oellerich, Thomas
A1  - Hartmann, Sylvia
T1  - T-cell-derived Hodgkin lymphoma has motility characteristics intermediate between Hodgkin and anaplastic large cell lymphoma
T2  - Journal of cellular and molecular medicine
N2  - Classic Hodgkin lymphoma (cHL) is usually characterized by a low tumour cell content, derived from crippled germinal centre B cells. Rare cases have been described in which the tumour cells show clonal T-cell receptor rearrangements. From a clinicopathological perspective, it is unclear if these cases should be classified as cHL or anaplastic large T-cell lymphoma (ALCL). Since we recently observed differences in the motility of ALCL and cHL tumour cells, here, we aimed to obtain a better understanding of T-cell-derived cHL by investigating their global proteomic profiles and their motility. In a proteomics analysis, when only motility-associated proteins were regarded, T-cell-derived cHL cell lines showed the highest similarity to ALK− ALCL cell lines. In contrast, T-cell-derived cHL cell lines presented a very low overall motility, similar to that observed in conventional cHL. Whereas all ALCL cell lines, as well as T-cell-derived cHL, predominantly presented an amoeboid migration pattern with uropod at the rear, conventional cHL never presented with uropods. The migration of ALCL cell lines was strongly impaired upon application of different inhibitors. This effect was less pronounced in cHL cell lines and almost invisible in T-cell-derived cHL. In summary, our cell line-derived data suggest that based on proteomics and migration behaviour, T-cell-derived cHL is a neoplasm that shares features with both cHL and ALCL and is not an ALCL with low tumour cell content. Complementary clinical studies on this lymphoma are warranted.
KW  - anaplastic large cell lymphoma
KW  - Hodgkin lymphoma
KW  - motility
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79351
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-793515
SN  - 1582-4934
N1  - This project was supported by the Deutsche Forschungsgemeinschaft (grant HA6145/6-1).
N1  - Open Access funding enabled and organized by Projekt DEAL.
N1  - The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE22 partner repository with the dataset identifier PXD031907 (https://www.ebi.ac.uk/pride). Raw data of movies are available on request from the corresponding author (several hundred Megabytes for each movie).
VL  - 26
IS  - 12
SP  - 3495
EP  - 3505
PB  - Wiley-Blackwell
CY  - Hoboken, NJ
ER  -