TY  - JOUR
A1  - Winkelmann, Anne Ria
A1  - Bankov, Katrin
A1  - Müller-von der Grün, Jens
A1  - Cinatl, Jindrich
A1  - Wild, Peter Johannes
A1  - Vallo, Stefan
A1  - Demes, Melanie Christin
T1  - The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods
T2  - BMC urology
N2  - Background: Routine human papillomavirus (HPV) testing is performed in cervival cancer and is required for classification of some head and neck cancers. In penile cancer a statement on HPV association of the carcinoma is required. In most cases p16 immunohistochemistry as a surrogate marker is applied in this setting. Since differing clinical outcomes for HPV positive and HPV negative tumors are described we await HPV testing to be requested more frequently by clinicians, also in the context of HPV vaccination, where other HPV subtypes are expected to emerge.
Method: Therefore, a cohort of archived, formalin-fixed paraffin embedded (FFPE) penile neoplasias was stained for p16 and thereafter tested for HPV infection status via PCR based methods. Additionally to Sanger sequencing, we chose LCD-Array technique (HPV 3.5 LCD-Array Kit, Chipron; LCD-Array) for the detection of HPV in our probes expecting a less time consuming and sensitive HPV test for our probes.
Results: We found that LCD-Array is a sensitive and feasible method for HPV testing in routine diagnostics applicable to FFPE material in our cohort. Our cohort of penile carcinomas and carcinomas in situ was associated with HPV infection in 61% of cases. We detected no significant association between HPV infection status and histomorphological tumor characteristics as well as overall survival.
Conclusions: We showed usability of molecular HPV testing on a cohort of archived penile carcinomas. To the best of our knowledge, this is the first study investigating LCD-Array technique on a cohort of penile neoplasias.
KW  - Urological oncology
KW  - Viral infection
KW  - Viral oncology
KW  - Screening
KW  - HPV infection
KW  - Penile carcinomas
KW  - Sanger Sequencing
KW  - p16
KW  - LCD-Array
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69639
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-696394
SN  - 1471-2490
N1  - The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
N1  - Open Access funding enabled and organized by Projekt DEAL. The study was financed by the Dr. Senckenberg Institute of Pathology, Frankfurt am Main.
VL  - 22
IS  - art. 10
SP  - 1
EP  - 12
PB  - BioMed Central
CY  - London
ER  -