TY  - JOUR
A1  - Lucaciu, Alexandra
A1  - Kuhn, Hannah
A1  - Trautmann, Sandra
A1  - Ferreirós Bouzas, Nerea
A1  - Steinmetz, Helmuth
A1  - Pfeilschifter, Josef
A1  - Brunkhorst, Robert
A1  - Pfeilschifter, Waltraud
A1  - Subburayalu, Julien
A1  - Vutukuri, Rajkumar
T1  - A sphingosine 1-phosphate gradient is linked to the cerebral recruitment of T helper and regulatory T helper cells during acute ischemic stroke
T2  - International journal of molecular sciences
N2  - Emerging evidence suggests a complex relationship between sphingosine 1-phosphate (S1P) signaling and stroke. Here, we show the kinetics of S1P in the acute phase of ischemic stroke and highlight accompanying changes in immune cells and S1P receptors (S1PR). Using a C57BL/6 mouse model of middle cerebral artery occlusion (MCAO), we assessed S1P concentrations in the brain, plasma, and spleen. We found a steep S1P gradient from the spleen towards the brain. Results obtained by qPCR suggested that cells expressing the S1PR type 1 (S1P1+) were the predominant population deserting the spleen. Here, we report the cerebral recruitment of T helper (TH) and regulatory T (TREG) cells to the ipsilateral hemisphere, which was associated with differential regulation of cerebral S1PR expression patterns in the brain after MCAO. This study provides insight that the S1P-S1PR axis facilitates splenic T cell egress and is linked to the cerebral recruitment of S1PR+ TH and TREG cells. Further insights by which means the S1P-S1PR-axis orchestrates neuronal positioning may offer new therapeutic perspectives after ischemic stroke.
KW  - sphingosine 1-phosphate
KW  - regulatory T helper cells
KW  - stroke
KW  - sphingosine kinase
KW  - sphingosine 1-phosphate receptor
KW  - ceramides
KW  - fingolimod
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55937
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-559372
SN  - 1422-0067
N1  - © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 21
IS  - 17, art. 6242
SP  - 1
EP  - 20
PB  - MDPI
CY  - Basel
ER  -