TY  - JOUR
A1  - Lodemann, Edgar
A1  - Drahovsky, Dusan
A1  - Flöhl, Rainer
A1  - Wacker, Adolf
T1  - Über die spezifische Bindung von TMG in E. coli : zum Mechanismus der Enzyminduktion
T2  - Zeitschrift für Naturforschung, B
N2  - Following treatment with the β-galactosidase inducer [methyl-3H] -thiogalactoside, an induceracceptor-complex was isolated from extracts of E. coli K 12 using DEAE cellulose chromatography. Enzymatic digestion with trypsin suggested that the inducer was bound to a protein component.
Specific radioactive peaks demonstrated acceptor activity in the inducible strains E. coli K 12 and ML 3, but different results were obtained using the non-inducible mutants ML 35, ML 308 and ML 309.
The potent inhibitor of TMG-induction, o-nitrophenylfucoside, reduced the radioactive acceptor peak and caused a similar inhibition of β-galactosidase synthesis, p-nitrophenylfucoside was ineffective.
Further evidence is presented for the in vitro formation of an inducer-acceptor-complex in cell free extracts of E. coli K 12.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74872
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-748721
SN  - 1865-7117
SN  - 0932-0776
VL  - 22
SP  - 301
EP  - 306
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -