TY  - JOUR
A1  - Sporbert, Anje
A1  - Cseresnyes, Zoltan
A1  - Heidbreder, Meike
A1  - Domaing, Petra
A1  - Hauser, Stefan
A1  - Kaltschmidt, Barbara
A1  - Kaltschmidt, Christian
A1  - Heilemann, Mike
A1  - Widera, Darius
T1  - Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots
T2  - PLoS One
N2  - This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/30050
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-300500
SN  - 1932-6203
N1  - Copyright: © 2013 Sporbert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (5):e64023
PB  - PLoS
CY  - Lawrence, Kan.
ER  -