TY  - JOUR
A1  - Kielholz, Tobias
A1  - Rohde, Felix
A1  - Jung, Nathalie
A1  - Windbergs, Maike
T1  - Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections
T2  - Scientific reports
N2  - The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical implementation remains hampered by the lack of efficient delivery approaches to infected wound tissue. In this study, bacteriophage-loaded electrospun fiber mats were successfully developed as next-generation wound dressings for the treatment of infected wounds. We employed a coaxial electrospinning approach, creating fibers with a protective polymer shell, enveloping bacteriophages in the core while maintaining their antimicrobial activity. The novel fibers exhibited a reproducible fiber diameter range and morphology, while the mechanical fiber properties were ideal for application onto wounds. Further, immediate release kinetics for the phages were confirmed as well as the biocompatibility of the fibers with human skin cells. Antimicrobial activity was demonstrated against Staphylococcus aureus and Pseudomonas aeruginosa and the core/shell formulation maintained the bacteriophage activity for 4 weeks when stored at − 20 °C. Based on these promising characteristics, our approach holds great potential as a platform technology for the encapsulation of bioactive bacteriophages to enable the translation of phage therapy into clinical application.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82621
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-826215
SN  - 2045-2322
VL  - 13
IS  - Article number: 8330
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - London
ER  -