TY  - JOUR
A1  - Ichikawa, Takehiko
A1  - Nakazato, Kenichi
A1  - Keller, Philipp J.
A1  - Kajiura-Kobayashi, Hiroko
A1  - Stelzer, Ernst H. K.
A1  - Mochizuki, Atsushi
A1  - Nonaka, Shigenori
T1  - Live imaging of whole mouse embryos during gastrulation : migration analyses of epiblast and mesodermal cells
T2  - PLoS One
N2  - During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan. The precise details of these movements, however, are sometimes elusive, because of the limitations in live imaging. To overcome this problem, we developed techniques to enable observation of living mouse embryos with digital scanned light sheet microscope (DSLM). The achieved deep and high time-resolution images of GFP-expressing nuclei and following 3D tracking analysis revealed the following findings: (i) Interkinetic nuclear migration (INM) occurs in the epiblast at embryonic day (E)6 and 6.5. (ii) INM-like migration occurs in the E5.5 embryo, when the epiblast is a monolayer and not yet pseudostratified. (iii) Primary driving force for INM at E6.5 is not pressure from neighboring nuclei. (iv) Mesodermal cells migrate not as a sheet but as individual cells without coordination.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/31426
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-314267
SN  - 1932-6203
N1  - Copyright: © 2013 Ichikawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (7):e64506
PB  - PLoS
CY  - Lawrence, Kan.
ER  -