TY  - JOUR
A1  - Fusco, Claudia Mariateresa
A1  - Desch, Kristina
A1  - Dörrbaum, Aline Ricarda
A1  - Wang, Mantian
A1  - Staab, Anja
A1  - Chan, Ivy C. W.
A1  - Vail, Eleanor
A1  - Villeri, Veronica
A1  - Langer, Julian D.
A1  - Schuman, Erin M.
T1  - Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins
T2  - Nature Communications
N2  - Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation was independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/85736
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-857367
SN  - 2041-1723
VL  - 12
IS  - Article number: 6127
PB  - Nature Publishing Group UK
CY  - London
ER  -