TY  - JOUR
A1  - Tietze, Andreas
A1  - Shi, Yan‐Ni
A1  - Kronenwerth, Max
A1  - Bode, Helge Björn
T1  - Nonribosomal peptides produced by minimal and engineered synthetases with terminal reductase domains
T2  - ChemBioChem
N2  - Nonribosomal peptide synthetases (NRPSs) use terminal reductase domains for 2‐electron reduction of the enzyme‐bound thioester releasing the generated peptides as C‐terminal aldehydes. Herein, we reveal the biosynthesis of a pyrazine that originates from an aldehyde‐generating minimal NRPS termed ATRed in entomopathogenic Xenorhabdus indica. Reductase domains were also investigated in terms of NRPS engineering and, although no general applicable approach was deduced, we show that they can indeed be used for the production of similar natural and unnatural pyrazinones.
KW  - aldehydes
KW  - natural products
KW  - nonribosomal peptide synthetases
KW  - NRPS engineering
KW  - reductases
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56556
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565565
SN  - 1439-7633
VL  - 21
IS  - 19
SP  - 2750
EP  - 2754
PB  - Wiley-VCH GmbH
CY  - Weinheim
ER  -