TY  - JOUR
A1  - Strassman, Alexander
A1  - Schnütgen, Frank
A1  - Dai, Qi
A1  - Jones, Jennifer C.
A1  - Gomez, Angela C.
A1  - Pitstick, Lenore
A1  - Holton, Nathan E.
A1  - Moskal, Russell
A1  - Leslie, Erin R.
A1  - Melchner, Harald von
A1  - Beier, David R.
A1  - Bjork, Bryan C.
T1  - Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping
T2  - Disease models & mechanisms
N2  - Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1IREScre deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.
KW  - Conditional gene trap
KW  - Cleft palate
KW  - Mandible
KW  - Micrognathia
KW  - Pierre Robin sequence
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43912
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-439128
SN  - 1754-8411
SN  - 1754-8403
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
VL  - 10
IS  - 7
SP  - 909
EP  - 922
PB  - Company of Biologists Limited
CY  - Cambridge
ER  -