TY  - JOUR
A1  - Fischer, Doris
A1  - Nold, Marcel Friedrich
A1  - Nold-Petry, Claudia A.
A1  - Furlan, Antonio
A1  - Veldman, Alex
T1  - Protein C preserves microcirculation in a model of neonatal septic shock
T2  - Vascular health and risk management
N2  - Objectives: Sepsis remains a disease with a high mortality in neonates. Microcirculatory impairment plays a pivotal role in the development of multiorgan failure in septic newborns. The hemodynamic effects of recombinant activated protein C (rhAPC) were tested in an animal model of neonatal septic shock focusing on intestinal microcirculation.
Materials and methods: Endotoxic shock was triggered by intravenous application of Escherichia coli lipopolysaccarides in newborn piglets. Thereafter, five animals received a continuous infusion of 24 µg/kg/h rhAPC, and five received vehicle for control. Over the course of three hours, intestinal microcirculation was assessed by intravital microscopy every 30 min. Macrocirculation and blood counts were monitored simultaneously.
Results: After a short hypotensive period in all animals, the arterial blood pressure returned to baseline in the rhAPC-treated piglets, whereas the hypotension became increasingly severe in the controls. By 90 min, mean blood pressure in the controls was significantly lower than in the treatment group. Similar observations were made regaring microcirculation. After an early impairment in all study animals, functional capillary density and intestinal microcirculatory red blood cell velocity and red blood cell flow recovered in the rhAPC group, but deteriorated further in the control piglets.
Conclusion: Recombinant activated protein C protects macro- and microcirculation from endotoxic shock.
KW  - protein C
KW  - neonatal septic shock
KW  - microcirculation
KW  - intravital microscopy
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/25540
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-255408
SN  - 1176-6344
SN  - 1178-2048
N1  - This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.
VL  - 5
SP  - 775
EP  - 781
PB  - Dove Medical Press
CY  - Albany, Auckland
ER  -