TY  - JOUR
A1  - Yin, Wenguang
A1  - Kim, Hyun-Taek
A1  - Wang, Shengpeng
A1  - Gunawan, Felix
A1  - Wang, Lei
A1  - Kishimoto, Keishi
A1  - Zhong, Hua
A1  - Roman, Dany
A1  - Preussner, Jens
A1  - Günther, Stefan
A1  - Graef, Viola
A1  - Büttner, Carmen
A1  - Grohmann, Beate
A1  - Looso, Mario
A1  - Morimoto, Mitsuru
A1  - Mardon, Graeme
A1  - Offermanns, Stefan
A1  - Stainier, Didier
T1  - The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis
T2  - Nature Communications
N2  - Tubulogenesis is essential for the formation and function of internal organs. One such organ is the trachea, which allows gas exchange between the external environment and the lungs. However, the cellular and molecular mechanisms underlying tracheal tube development remain poorly understood. Here, we show that the potassium channel KCNJ13 is a critical modulator of tracheal tubulogenesis. We identify Kcnj13 in an ethylnitrosourea forward genetic screen for regulators of mouse respiratory organ development. Kcnj13 mutants exhibit a shorter trachea as well as defective smooth muscle (SM) cell alignment and polarity. KCNJ13 is essential to maintain ion homeostasis in tracheal SM cells, which is required for actin polymerization. This process appears to be mediated, at least in part, through activation of the actin regulator AKT, as pharmacological increase of AKT phosphorylation ameliorates the Kcnj13-mutant trachea phenotypes. These results provide insight into the role of ion homeostasis in cytoskeletal organization during tubulogenesis.
KW  - Cytoskeleton
KW  - Morphogenesis
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46890
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-468909
SN  - 2041-1723
N1  - Rights and permissions: Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 9
IS  - 1, Art. 2815
SP  - 1
EP  - 13
PB  - Nature Publishing Group UK
CY  - [London]
ER  -