TY  - JOUR
A1  - Banning, Antje
A1  - Babuke, Tanja
A1  - Kurrle, Nina Susanne
A1  - Meister, Melanie
A1  - Ruonala, Mika O.
A1  - Tikkanen, Ritva
T1  - Flotillins regulate focal adhesions by interacting with α-actinin and by influencing the activation of focal adhesion kinase
T2  - Cells
N2  - Cell–matrix adhesion and cell migration are physiologically important processes that also play a major role in cancer spreading. In cultured cells, matrix adhesion depends on integrin-containing contacts such as focal adhesions. Flotillin-1 and flotillin-2 are frequently overexpressed in cancers and are associated with poor survival. Our previous studies have revealed a role for flotillin-2 in cell–matrix adhesion and in the regulation of the actin cytoskeleton. We here show that flotillins are important for cell migration in a wound healing assay and influence the morphology and dynamics of focal adhesions. Furthermore, anchorage-independent growth in soft agar is enhanced by flotillins. In the absence of flotillins, especially flotillin-2, phosphorylation of focal adhesion kinase and extracellularly regulated kinase is diminished. Flotillins interact with α-actinin, a major regulator of focal adhesion dynamics. These findings are important for understanding the molecular mechanisms of how flotillin overexpression in cancers may affect cell migration and, especially, enhance metastasis formation.
KW  - focal adhesion
KW  - cancer
KW  - metastasis
KW  - cell migration
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51518
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-515182
N1  - © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 7
IS  - 28
PB  - MDPI
CY  - Basel
ER  -