TY  - JOUR
A1  - Wang, Xuzhu
A1  - Zhang, Yufeng
A1  - Choukroun, Joseph
A1  - Ghanaati, Shahram Michael
A1  - Miron, Richard J.
T1  - Behavior of gingival fibroblasts on titanium implant surfaces in combination with either Injectable-PRF or PRP
T2  - International journal of molecular sciences
N2  - Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient’s own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β, collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highest in the i-PRF group. These findings demonstrate that liquid platelet concentrates can be formulated without the use of anticoagulants and present much translational potential for future research. Future animal and clinical trials are now necessary to further investigate the potential of utilizing i-PRF for soft tissue regenerative protocols in combination with various biomaterials.
KW  - fibrin
KW  - blood
KW  - platelets
KW  - regeneration
KW  - wound healing
KW  - fibroblasts
KW  - platelet-rich fibrin
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56320
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-563209
SN  - 1422-0067
SN  - 1661-6596
VL  - 18
IS  - 331
ER  -