TY  - JOUR
A1  - Hoffmeister, Mascha
A1  - Maier, Wolfgang
A1  - Thines, Marco
A1  - Becker, Yvonne
T1  - Tracking host infection and reproduction of Peronospora salviae‐officinalis using an improved method for confocal laser scanning microscopy
T2  - Plant pathology
N2  - Peronospora salviae‐officinalis, the causal agent of downy mildew on common sage, is an obligate biotrophic pathogen. It grows in the intercellular spaces of the leaf tissue of sage and forms intracellular haustoria to interface with host cells. Although P. salviae‐officinalis was described as a species of its own 10 years ago, the infection process remains obscure. To address this, a histological study of various infection events, from the adhesion of conidia on the leaf surface to de novo sporulation is presented here. As histological studies of oomycetes are challenging due to the lack of chitin in their cell wall, we also present an improved method for staining downy mildews for confocal laser scanning microscopy as well as evaluating the potential of autofluorescence of fixed nonstained samples. For staining, a 1:1 mixture of aniline blue and trypan blue was found most suitable and was used for staining of oomycete and plant structures, allowing discrimination between them as well as the visualization of plant immune responses. The method was also used to examine samples of Peronospora lamii on Lamium purpureum and Peronospora belbahrii on Ocimum basilicum, demonstrating the potential of the presented histological method for studying the infection processes of downy mildews in general.
KW  - aniline blue
KW  - confocal laser scanning microscopy
KW  - Peronospora belbahrii
KW  - Peronospora lamii
KW  - Peronospora salviae-officinalis
KW  - trypan blue
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56548
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565481
SN  - 1365-3059
VL  - 69
IS  - 5
SP  - 922
EP  - 931
PB  - John Wiley & Sons Ltd
CY  - Oxford
ER  -