TY  - JOUR
A1  - Alt, Katharina
A1  - Feldmeyer, Barbara
A1  - Kochmann, Judith
A1  - Klimpel, Sven
T1  - Gene expression and allergenic potential of Pseudoterranova bulbosa L3 from different infection sites in North Atlantic cod (Gadus morhua)
T2  - Journal of fish diseases
N2  - The recent advances in molecular methods and data processing have facilitated research on anisakid nematodes. While most research efforts were made regarding the genus Anisakis, since this genus is held responsible for the majority of reported clinical signs, there is still a demand for data on the genus Pseudoterranova. Several case studies of severe invasive anisakidosis affecting various organs caused by species of the P. decipiens complex have been described. To better understand the way these parasites might infest their fish host, we examined whether parasite location within the fish host affects gene expression. A de novo assembly of the transcriptome of Pseudoterranova bulbosa, isolated from North Atlantic cod, was analysed for patterns of differential gene expression between samples taken from liver and viscera. We additionally searched for homologs to known nematode allergens, to give a first estimate of the potential allergenicity of P. bulbosa. There was a subtle difference in the gene expression of samples taken from liver and viscera. Seventy genes were differentially expressed, 32 genes were upregulated in parasites isolated from liver and 38 genes were upregulated in parasites from viscera. Homologs of five nematode allergens were identified among the genes expressed by P. bulbosa. Our transcriptome of P. bulbosa will be a valuable resource for further meta-analyses and resequencing projects.
KW  - Anisakidae
KW  - differentially expressed genes
KW  - human pathogenic Nematoda
KW  - seafood allergy
KW  - transcriptome
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79166
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-791668
SN  - 1365-2761
N1  - Open access funding enabled and organized by ProjektDEAL.
N1  - The present study is a result of the Centre for Translational Biodiversity Genomics (LOEWE-TBG) and was supported through the  program  LOEWE- Landes- Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz of Hesse's Ministry of Higher Education, Research and the Arts
VL  - 45
IS  - 8
SP  - 1073
EP  - 1086
PB  - Wiley-Blackwell
CY  - Oxford [u.a.]
ER  -