TY  - JOUR
A1  - Kreß, Aljoscha
A1  - Kuch, Ulrich
A1  - Oehlmann, Jörg
A1  - Müller, Ruth
T1  - Effects of diapause and cold acclimation on egg ultrastructure : new insights into the cold hardiness mechanisms of the Asian tiger mosquito Aedes (Stegomyia) albopictus
T2  - Journal of vector ecology
N2  - The Asian tiger mosquito, Aedes albopictus (Diptera: Culicidae, SKUSE), is an important threat to public health due to its rapid spread and its potential as a vector. The eggs of Ae. albopictus are the most cold resistant life stage and thus, the cold hardiness of eggs is used to predict the future occurrence of the species in distribution models. However, the mechanism of cold hardiness has yet to be revealed. To address this question, we analyzed the layers of diapausing and cold acclimatized eggs of a temperate population of Ae. albopictus in a full factorial test design using transmission electron microscopy. We reviewed the hypotheses that a thickened wax layer or chorion is the cause of cold hardiness but found no evidence. As a result of the induced diapause, the thickness of the dark endochorion as a layer of high electron density and thus an assumed location for waxes was decreasing. We therefore hypothesized a qualitative alteration of the wax layer due to compaction. Cold acclimation was causing an increase in the thickness of the middle serosa cuticle indicating a detachment of serosa membrane from the endochorion as a potential adaptation strategy to isolate inoculating ice formations in the inter-membranous space.
KW  - Chorion
KW  - cold tolerance
KW  - compaction
KW  - freeze avoidance
KW  - wax layer
KW  - winter survival
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45113
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-451139
SN  - 1948-7134
SN  - 1081-1710
N1  - © 2016 The Society for Vector Ecology
VL  - 41
IS  - 1
SP  - 142
EP  - 150
PB  - Wiley Interscience
CY  - Hoboken, NJ [u. a.]
ER  -