TY  - JOUR
A1  - Scheider, Jessica
A1  - Afonso-Grunz, Fabian
A1  - Jessl, Luzie
A1  - Hoffmeier, Klaus
A1  - Winter, Peter
A1  - Oehlmann, Jörg
T1  - Morphological and transcriptomic effects of endocrine modulators
on the gonadal differentiation of chicken embryos: the case
of tributyltin (TBT)
T2  - Toxicology Letters
N2  - Morphological malformations induced by tributyltin (TBT) exposure during embryonic development have already been characterized in various taxonomic groups, but, nonetheless, the molecular processes underlying these changes remain obscure. The present study provides the first genome-wide screening for differentially expressed genes that are linked to morphological alterations of gonadal tissue from chicken embryos after exposure to TBT. We applied a single injection of TBT (between 0.5 and 30 pg as Sn/g egg) into incubated fertile eggs to simulate maternal transfer of the endocrine disruptive compound. Methyltestosterone (MT) served as a positive control (30 pg/g egg). After 19 days of incubation, structural features of the gonads as well as genome-wide gene expression profiles were assessed simultaneously. TBT induced significant morphological and histological malformations of gonadal tissue from female embryos that show a virilization of the ovaries. This phenotypical virilization was mirrored by altered expression profiles of sex-dependent genes. Among these are several transcription and growth factors (e.g. FGF12, CTCF, NFIB), whose altered expression might serve as a set of markers for early identification of endocrine active chemicals that affect embryonic development by transcriptome profiling without the need of elaborate histological analyses.
KW  - Endocrine disruption
KW  - Gonads
KW  - Development
KW  - Testosterone
KW  - Androgen
KW  - Sex reversal
KW  - Masculinization
KW  - Ovary
KW  - Testis
KW  - Chicken
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56547
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565477
SN  - 0378-4274
SN  - 1879-3169
VL  - 284.2018
SP  - 143
EP  - 151
PB  - Elsevier Science
CY  - Amsterdam [u.a.]
ER  -