TY  - CHAP
A1  - Rozanova, Svitlana
A1  - Barkovits, Katalin
A1  - Nikolov, Miroslav
A1  - Schmidt, Carla
A1  - Urlaub, Henning
A1  - Marcus, Katrin
A2  - Marcus, Katrin
A2  - Eisenacher, Martin
A2  - Sitek, Barbara
T1  - Quantitative mass spectrometry-based proteobmics: an overview
T2  - Quantitative methods in proteomics / edited by Katrin Marcus, Martin Eisenacher, Barbara Sitek ; Methods in molecular biology ; 2228
N2  - In recent decades, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review actual strategies and problems of the methods for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.
KW  - Mass spectrometry
KW  - Proteomics
KW  - Relative quantification
KW  - Absolute quantification
KW  - Label-free
KW  - Isotope labeling
KW  - Metabolic labeling
KW  - Chemical labeling
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69767
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-697677
SN  - 978-1-0716-1023-7
SN  - 978-1-0716-1026-8
SN  - 978-1-0716-1024-4
SN  - 1940-6029
SN  - 1064-3745
N1  - The authors were supported by P.U.R.E. (Protein Research Unit Ruhr within Europe) and ProDi (Center for protein diagnostics), Ministry of Innovation, Science and Research of North-Rhine Westphalia, Germany, and by the H2020 project NISCI, (GA no. 681094) and by the ISF program of the European Union.
SP  - 85
EP  - 116
PB  - Humana Press ; [Springer]
CY  - Totowa, NJ ; [Heidelberg]
ER  -