TY  - INPR
A1  - Taniguchi, Reiya
A1  - Orniacki, Clarisse
A1  - Kreysing, Jan Philipp
A1  - Zila, Vojtech
A1  - Zimmerli, Christian Eugen
A1  - Böhm, Stefanie
A1  - Turoňová, Beata
A1  - Kräusslich, Hans-Georg
A1  - Doye, Valérie
A1  - Beck, Martin
T1  - Nuclear pores safeguard the integrity of the nuclear envelope
T2  - bioRxiv
N2  - Nuclear pore complexes (NPCs) constitute giant channels within the nuclear envelope that mediate nucleocytoplasmic exchange. NPC diameter is thought to be regulated by nuclear envelope tension, but how such diameter changes are physiologically linked to cell differentiation, where mechanical properties of nuclei are remodeled and nuclear mechanosensing occurs, remains unstudied. Here we used cryo-electron tomography to show that NPCs dilate during differentiation of mouse embryonic stem cells into neural progenitors. In Nup133-deficient cells, which are known to display impaired neural differentiation, NPCs however fail to dilate. By analyzing the architectures of individual NPCs with template matching, we revealed that the Nup133-deficient NPCs are structurally heterogeneous and frequently disintegrate, resulting in the formation of large nuclear envelope openings. We propose that the elasticity of the NPC scaffold mechanically safeguards the nuclear envelope. Our studies provide a molecular explanation for how genetic perturbation of scaffolding components of macromolecular complexes causes tissue-specific phenotypes.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83055
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-830554
UR  - https://www.biorxiv.org/content/10.1101/2024.02.05.578890v1
IS  - 2024.02.05.578890 Version 1
PB  - bioRxiv
ER  -