TY  - JOUR
A1  - Davies, Karen M.
A1  - Blum, Thorsten
A1  - Kühlbrandt, Werner
T1  - Conserved in situ arrangement of complex I and III2 in mitochondrial respiratory chain supercomplexes of mammals, yeast, and plants
T2  - Proceedings of the National Academy of Sciences of the United States of America
N2  - We used electron cryo-tomography and subtomogram averaging to investigate the structure of complex I and its supramolecular assemblies in the inner mitochondrial membrane of mammals, fungi, and plants. Tomographic volumes containing complex I were averaged at ∼4 nm resolution. Principal component analysis indicated that ∼60% of complex I formed a supercomplex with dimeric complex III, while ∼40% were not associated with other respiratory chain complexes. The mutual arrangement of complex I and III2 was essentially conserved in all supercomplexes investigated. In addition, up to two copies of monomeric complex IV were associated with the complex I1III2 assembly in bovine heart and the yeast Yarrowia lipolytica, but their positions varied. No complex IV was detected in the respiratory supercomplex of the plant Asparagus officinalis. Instead, an ∼4.5-nm globular protein density was observed on the matrix side of the complex I membrane arm, which we assign to γ-carbonic anhydrase. Our results demonstrate that respiratory chain supercomplexes in situ have a conserved core of complex I and III2, but otherwise their stoichiometry and structure varies. The conserved features of supercomplex assemblies indicate an important role in respiratory electron transfer.
KW  - respirasomes
KW  - respiratory chain supercomplexes
KW  - cryo-electron tomography
KW  - subtomogram averaging
KW  - mitochondria
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/27616
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-276164
SN  - 1091-6490
SN  - 0027-8424
N1  - This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
VL  - 115
IS  - Art. 20172070
SP  - 1
EP  - 6
PB  - National Acad. of Sciences
CY  - Washington, DC
ER  -