TY  - JOUR
A1  - Bissen, Diane
A1  - Kracht, Maximilian Ken
A1  - Foß, Franziska
A1  - Acker-Palmer, Amparo
T1  - Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution
T2  - STAR Protocols
N2  - Highlights
• Enables immunostaining and visualization of epitopes deep within brain slices
• Utilizes expansion microscopy to increase imaging resolution
• Optimized for brain organotypic slice cultures and tested in acute brain slices
• Analysis workflow for protein distribution (surface vs. intracellular pool) using Imaris
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Summary
Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs intracellular distribution of AMPA receptors at super-resolution during homeostatic plasticity. We have optimized the protocol for brain organotypic slice culture and tested in acute brain slices. This protocol is suitable to study protein distribution under multiple plasticity paradigms.
KW  - Cell Biology
KW  - Microscopy
KW  - Neuroscience
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78366
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-783663
SN  - 2666-1667
VL  - 3
IS  - 3, 101507
SP  - 1
EP  - 24
PB  - Elsevier
CY  - Amsterdam
ER  -