TY  - INPR
A1  - Bozhüyük, Kenan A. J.
A1  - Watzel, Jonas
A1  - Abbood, Nadya
A1  - Bode, Helge Björn
T1  - Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases
T2  - bioRxiv
N2  - Non-ribosomal peptide synthetases (NRPSs) are the origin of a wide range of natural products, including many clinically used drugs. Engineering of these often giant biosynthetic machineries to produce novel non-ribosomal peptides (NRPs) at high titre is an ongoing challenge. Here we describe a strategy to functionally combine NRPS fragments of Gram-negative and -positive origin, synthesising novel peptides at titres up to 290 mg l-1. Extending from the recently introduced definition of eXchange Units (XUs), we inserted synthetic zippers (SZs) to split single protein NRPSs into up to three independently expressed and translated polypeptide chains. These synthetic type of NRPS (type S) enables easier access to engineering, overcomes cloning limitations, and provides a simple and rapid approach to building peptide libraries via the combination of different NRPS subunits.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72744
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-727448
IS  - 2020.05.06.080655
ER  -