TY  - JOUR
A1  - Kupka, Johannes Raphael
A1  - Kohler, Annika
A1  - El Bagdadi, Karima
A1  - Bostelmann, Richard Klaus Ludwig
A1  - Brenneis, Marco
A1  - Fleege, Christoph
A1  - Chan, Danny
A1  - Zaucke, Frank
A1  - Meurer, Andrea
A1  - Rickert, Marcus
A1  - Jenei-Lanz, Zsuzsa
T1  - Adrenoceptor expression during intervertebral disc degeneration
T2  - International journal of molecular sciences
N2  - Healthy and degenerating intervertebral discs (IVDs) are innervated by sympathetic nerves, however, adrenoceptor (AR) expression and functionality have never been investigated systematically. Therefore, AR gene expression was analyzed in both tissue and isolated cells from degenerated human IVDs. Furthermore, human IVD samples and spine sections of wildtype mice (WT) and of a mouse line that develops spontaneous IVD degeneration (IVDD, in SM/J mice) were stained for ARs and extracellular matrix (ECM) components. In IVD homogenates and cells α1a-, α1b-, α2a-, α2b-, α2c-, β1-, and β2-AR genes were expressed. In human sections, β2-AR was detectable, and its localization parallels with ECM alterations. Similarly, in IVDs of WT mice, only β2-AR was expressed, and in IVDs of SM/J mice, β2AR expression was stronger accompanied by increased collagen II, collagen XII, decorin as well as decreased cartilage oligomeric matrix protein expression. In addition, norepinephrine stimulation of isolated human IVD cells induced intracellular signaling via ERK1/2 and PKA. For the first time, the existence and functionality of ARs were demonstrated in IVD tissue samples, suggesting that the sympathicus might play a role in IVDD. Further studies will address relevant cellular mechanisms and thereby help to develop novel therapeutic options for IVDD.
KW  - intervertebral disc (IVD)
KW  - IVD degeneration (IVDD)
KW  - sympathicus
KW  - adrenoceptors
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54431
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-544314
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 21
IS  - 2085
PB  - MDPI
CY  - Basel
ER  -