TY  - JOUR
A1  - Pérez, Alexander José
A1  - Bode, Helge Björn
T1  - ω-Azido fatty acids as probes to detect fatty acid biosynthesis, degradation, and modification
T2  - Journal of lipid research
N2  - FAs play a central role in the metabolism of almost all known cellular life forms. Although GC-MS is regarded as a standard method for FA analysis, other methods, such as HPLC/MS, are nowadays widespread but are rarely applied to FA analysis. Here we present azido-FAs as probes that can be used to study FA biosynthesis (elongation, desaturation) or degradation (β-oxidation) upon their uptake, activation, and metabolic conversion. These azido-FAs are readily accessible by chemical synthesis and their matization with high sensitivity by HPLC/MS, contributing a powerful tool to FA analysis, and hence, lipid analysis in general.
KW  - click-chemistry
KW  - β-oxidation
KW  - fatty acid metabolism
KW  - fatty acid desaturation
KW  - strained promoted cycloadditon
KW  - cyclooctyne
KW  - azido-fatty acids
KW  - S-azidoacyl-N-acetylcysteamine
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76969
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-769695
SN  - 0022-2275
VL  - 55.2014
IS  - 9
SP  - 1897
EP  - 1901
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -