TY  - JOUR
A1  - Siegert, Sandra
A1  - Thaler, Sonja
A1  - Wagner, Ralf
A1  - Schnierle, Barbara
T1  - Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
T2  - AIDS Research and Therapy
N2  - Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.
KW  - HIV-1
KW  - MLV/HIV-1
KW  - pseudotyped vectors
Y1  - 2005
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/2789
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-26194
UR  - http://www.aidsrestherapy.com/content/2/1/7
N1  - © 2005 Siegert et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2
IS  - 7
ER  -