TY  - JOUR
A1  - Dixit, Sameer
A1  - Müller-McNicoll, Michaela
A1  - David, Vojtěch
A1  - Zarnack, Katharina
A1  - Ule, Jernej
A1  - Hashimi, Hassan
A1  - Lukeš, Julius
T1  - Differential binding of mitochondrial transcripts by MRB8170 and MRB4160 regulates distinct editing fates of mitochondrial mRNA in trypanosomes
T2  - mBio
N2  - A dozen mRNAs are edited by multiple insertions and/or deletions of uridine residues in the mitochondrion of Trypanosoma brucei. Several protein complexes have been implicated in performing this type of RNA editing, including the mitochondrial RNA-binding complex 1 (MRB1). Two paralogous novel RNA-binding proteins, MRB8170 and MRB4160, are loosely associated with the core MRB1 complex. Their roles in RNA editing and effects on target mRNAs are so far not well understood. In this study, individual-nucleotide-resolution UV-cross-linking and affinity purification (iCLAP) revealed a preferential binding of both proteins to mitochondrial mRNAs, which was positively correlated with their extent of editing. Integrating additional in vivo and in vitro data, we propose that binding of MRB8170 and/or MRB4160 onto pre-mRNA marks it for the initiation of editing and that initial binding of both proteins may facilitate the recruitment of other components of the RNA editing/processing machinery to ensure efficient editing. Surprisingly, MRB8170 also binds never-edited mRNAs, suggesting that at least this paralog has an additional role outside RNA editing to shape the mitochondrial transcriptome.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44627
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-446275
SN  - 2150-7511
SN  - 2161-2129
N1  - Copyright © 2017 Dixit et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
VL  - 8
IS  - 1, e02288-16
SP  - 1
EP  - 15
PB  - American Society for Microbiology
CY  - Washington, DC
ER  -