TY  - JOUR
A1  - Orlowska, Anna
A1  - Perera, Pallale Tharushi
A1  - Al Kobaisi, Mohammad
A1  - Dias, Andre
A1  - Nguyen, Huu Khuong Duy
A1  - Ghanaati, Shahram Michael
A1  - Baulin, Vladimir
A1  - Crawford, Russell J.
A1  - Ivanova, Elena P.
T1  - The effect of coatings and nerve growth factor on attachment and differentiation of pheochromocytoma cells
T2  - Materials
N2  - Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
KW  - nerve growth factor (NGF)
KW  - poly-L-lysine
KW  - fibronectin
KW  - laminin
KW  - PC12 neuronal differentiation
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45337
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-453370
SN  - 1996-1944
N1  - © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 11
IS  - 1, Art. 60
SP  - 1
EP  - 10
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -