TY  - JOUR
A1  - Gold, Vicki A. M.
A1  - Chroscicki, Piotr
A1  - Bragoszewski, Piotr
A1  - Chacinska, Agnieszka
T1  - Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo‐tomography
T2  - EMBO reports
N2  - We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
KW  - electron cryo-tomography
KW  - mitochondrial protein import
KW  - ribosomes
KW  - TOM complex
KW  - translation
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46577
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-465779
SN  - 1469-3178
SN  - 1469-221X
N1  - License: This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
VL  - 18
IS  - 10
SP  - 1786
EP  - 1800
PB  - EMBO Press ; Wiley
CY  - Heidelberg ;  Hoboken, NJ [u. a.]
ER  -