TY  - JOUR
A1  - Wang, Lina
A1  - Hu, Lei
A1  - Grygorczyk, Ryszard
A1  - Shen, Xueyong
A1  - Schwarz, Wolfgang
T1  - Modulation of extracellular atp content of mast cells and drg neurons by irradiation : studies on underlying mechanism of low-level-laser therapy
T2  - Mediators of inflammation
N2  - Low-level-laser therapy (LLLT) is an effective complementary treatment, especially for anti-inflammation and wound healing in which dermis or mucus mast cells (MCs) are involved. In periphery, MCs crosstalk with neurons via purinergic signals and participate in various physiological and pathophysiological processes. Whether extracellular ATP, an important purine in purinergic signaling, of MCs and neurons could be modulated by irradiation remains unknown. In this study, effects of red-laser irradiation on extracellular ATP content of MCs and dorsal root ganglia (DRG) neurons were investigated and underlying mechanisms were explored in vitro. Our results show that irradiation led to elevation of extracellular ATP level in the human mast cell line HMC-1 in a dose-dependent manner, which was accompanied by elevation of intracellular ATP content, an indicator for ATP synthesis, together with [Ca2+]i elevation, a trigger signal for exocytotic ATP release. In contrast to MCs, irradiation attenuated the extracellular ATP content of neurons, which could be abolished by ARL 67156, a nonspecific ecto-ATPases inhibitor. Our results suggest that irradiation potentiates extracellular ATP of MCs by promoting ATP synthesis and release and attenuates extracellular ATP of neurons by upregulating ecto-ATPase activity. The opposite responses of these two cell types indicate complex mechanisms underlying LLLT.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50643
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-506435
SN  - 1466-1861
SN  - 0962-9351
N1  - Copyright © 2015 Lina Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2015
IS  - Art. 630361
SP  - 1
EP  - 9
PB  - Hindawi Publishing Corp.
CY  - Sylvania, Ohio
ER  -