TY  - JOUR
A1  - Zhou, Hai
A1  - Zeng, Zhen
A1  - Köntgen, Frank
A1  - Khan, Mona
A1  - Mombaerts, Peter
T1  - The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation
T2  - Genesis
N2  - Spermatogonial stem cells (SSCs) are adult stem cells that are slowly cycling and self-renewing. The pool of SSCs generates very large numbers of male gametes throughout the life of the individual. SSCs can be cultured in vitro for long periods of time, and established SSC lines can be manipulated genetically. Upon transplantation into the testes of infertile mice, long-term cultured mouse SSCs can differentiate into fertile spermatozoa, which can give rise to live offspring. Here, we show that the testicular soma of mice with a conditional knockout (conKO) in the X-linked gene Tsc22d3 supports spermatogenesis and germline transmission from cultured mouse SSCs upon transplantation. Infertile males were produced by crossing homozygous Tsc22d3 floxed females with homozygous ROSA26-Cre males. We obtained 96 live offspring from six long-term cultured SSC lines with the aid of intracytoplasmic sperm injection. We advocate the further optimization of Tsc22d3-conKO males as recipients for testis transplantation of SSC lines.
KW  - germline stem cell
KW  - GILZ
KW  - spermatogenesis
KW  - SSC
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/53321
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-533218
SN  - 1526-968X
SN  - 1526-954X
N1  - This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
VL  - 57
IS  - 6, e23295
SP  - 1
EP  - 10
PB  - Wiley
CY  - Chichester [u. a.]
ER  -