TY  - JOUR
A1  - Brol, Maximilian
A1  - Georgiou, Stella
A1  - Rasmussen, Ditlev Nytoft
A1  - Ortiz, Cristina
A1  - Klein, Sabine
A1  - Schierwagen, Robert
A1  - Uschner, Frank Erhard
A1  - Eberle, Larissa
A1  - Detlefsen, Sönke
A1  - Pantazopoulou, Vasiliki I.
A1  - Thiele, Maja
A1  - Filippa, Vasiliki
A1  - Torres Núñez, Sandra
A1  - Anastasiadou, Ema
A1  - Krag, Aleksander
A1  - Trebicka, Jonel
T1  - Effects of ethanol feeding in early-stage NAFLD mice induced by western diet
T2  - Livers
N2  - Background: The prevalence of metabolic liver diseases is increasing and approved pharmacological treatments are still missing. Many animal models of nonalcoholic fatty liver disease (NAFLD) show a full spectrum of fibrosis, inflammation and steatosis, which does not reflect the human situation since only up to one third of the patients develop fibrosis and nonalcoholic steatohepatitis (NASH). Methods: Seven week old C57Bl/J mice were treated with ethanol, Western diet (WD) or both. The animals’ liver phenotypes were determined through histology, immunohistochemistry, Western blotting, hepatic triglyceride content and gene expression levels. In a human cohort of 80 patients stratified by current alcohol misuse and body mass index, liver histology and gene expression analysis were performed. Results: WD diet and ethanol-treated animals showed severe steatosis, with high hepatic triglyceride content and upregulation of fatty acid synthesis. Mild fibrosis was revealed using Sirius-red stains and gene expression levels of collagen. Inflammation was detected using histology, immunohistochemistry and upregulation of proinflammatory genes. The human cohort of obese drinkers showed similar upregulation in genes related to steatosis, fibrosis and inflammation. Conclusions: We provide a novel murine model for early-stage fatty liver disease suitable for drug testing and investigation of pathophysiology.
KW  - ALD
KW  - NAFLD
KW  - NASH
KW  - metabolic liver disease
KW  - liver steatosis
KW  - animal model
KW  - mouse
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/60989
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-609897
SN  - 2673-4389
VL  - 1
IS  - 1
SP  - 27
EP  - 39
PB  - MDPI
CY  - Basel
ER  -