TY - JOUR A1 - Ben Marnissi, Jamila A1 - Cesari, Michele A1 - Rebecchi, Lorena A1 - Bertolani, Roberto T1 - Integrative description of a new Tunisian tardigrade species, Macrobiotus azzunae sp. nov. (Eutardigrada, Macrobiotidae, hufelandi group) T2 - European journal of taxonomy N2 - In this paper a new tardigrade species, Macrobiotus azzunae sp. nov., from Tunisia, is described. An integrative taxonomic approach was applied by combining morphological, morphometric and molecular data. In particular, light and scanning electron microscopy observations, and four genetic markers, three nuclear (18S rRNA, 28S rRNA and ITS-2) and one mitochondrial (COI) were used. The analysis showed that M. azzunae sp. nov. belongs to the Macrobiotus hufelandi group and is most similar to Macrobiotus sandrae Bertolani & Rebecchi, 1993. It differs from M. sandrae by a more pronounced constriction of the first macroplacoid (hardly visible in M. sandrae) and for the eggshell shape, with thinner wires of the reticulum and meshes around the processes larger than the inter-process meshes in M. azzunae sp. nov., while all meshes are similar in size in M. sandrae. The species is gonochoristic. With this discovery, there are 33 species of tardigrades identified in Tunisia, all non-marine. This result, compared with nearby Sicily, where more research has been conducted, indicates that there is a considerable potential for identification of new species. Further research will be most informative if multiple habitats are explored and if carried out with an integrated approach as done in this present work. T3 - European journal of taxonomy : EJT - 758:122–146 KW - Tardigrada KW - Ain Soltan Forest KW - Tunisia KW - Macrobiotus azzunae KW - new species Y1 - 2021 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61365 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-613656 SN - 2118-9773 VL - 2021 IS - 758 SP - 122 EP - 146 PB - Muséum National d'Histoire Naturelle CY - Paris ER -